Dr. Thelma Abeysinghe

Senior Lecturer (G II)


Chemistry
Faculty of Natural Sciences
Website RG GS
Contact:

Central Campus, Room No:314, 1st Floor, S& T Building, The Open University of Sri Lanka, Nawala, Nugegoda

Office: 112881322, Ext:

E-mail: dtabe@ou.ac.lk

Web: https://www.linkedin.com/feed/?trk=

Last Updated on 3 years

Areas of Specialization:

Biochemistry and Molecular Biology, Organic Chemistry

Research interests:

Pesticide Absorption by Plants, Food Chemistry

Affiliations:

NULL

Course Coordination:

CYU5303, CYU5306, CYU6608

Awards:

McCloskey Research Award by the University of Iowa, USA                                              2013

 

Graduate College Summer Fellowship Award by the University of Iowa, USA                  2013

 

Spring Travel Grant Award from the Executive Council of Graduate & Professional          2014

Students (ECGPS), University of Iowa, USA

 

Spring Travel Grant Award from the Graduate Students Senate (GSS),                               2014

University of Iowa, USA

 

 

Researches:

1)         Somarathna, T., Fernando, W.M.A.D., Ranaweera, K., Premakumara, G.A., Abeysinghe,T., Weerakkody, N. Antimicrobial activity and phytochemical screening of Alpinia malaccensis   (Ran-kiriya) against food-borne bacteria, J. of Appl. Microbiol.,  doi: 10.1111/jam.14039

 

(2)        Thelma Abeysinghe, B. Hong, Zhen Wang, Amnon Kohen. (2016) Preserved Hydride Transfer Mechanism in Evolutionary Divergent Thymidylate Synthases, Current Topics in Biochem. Res., 17, 19-30

 

(3)        Thelma Abeysinghe, Bradley T. Falk, Catherine Suchanek, Andrew L. Lee and Amnon Kohen, Altered Protein Motions Coupled to the Chemical Step in Heavy Thymidylate Synthase (Manuscript in preparation for Journal of American chemical Society, JACS)

 

(4)        Abeysinghe, Thelma and Kohen, A. (2015) Role of Long-Range Protein Dynamics in Different Thymidylate Synthase Catalyzed Reactions, International Journal of Molecular Sciences (IJMS), 16, 7304-7319.

 

(5)        Ghosh, A. K., Islam, Z., Krueger, J., Abeysinghe, Thelma and Kohen, A. (2015) The general base in the thymidylate synthase catalyzed proton abstraction, Physical Chemistry Chemical Physics (PCCP), 17, 30867-30875

 

(6)       Singh, P.*, Abeysinghe, Thelma.* and Kohen, A. (2015) Linking Protein Motion to Enzyme Catalysis, Molecules, 20, 1192-1209.

* – Author’s equal contribution

 

(7)        Wang, Z., Sapienza, P. J., Abeysinghe, Thelma., Luzum, C., Lee, A. L., Finer-Moore, J. S., Stroud, R. M., and Kohen, A. (2013) Mg2+ Binds to the Surface of Thymidylate Synthase and Affects Hydride Transfer at the Interior Active Site, Journal of American chemical Society, (JACS)135, 7583-7592.

 

(8)        Wang, Z.*, Abeysinghe, Thelma.*, Finer-Moore, J. S., Stroud, R. M., and Kohen, A. (2012) A Remote Mutation Affects the Hydride Transfer by Disrupting Concerted Protein Motions in Thymidylate Synthase, Journal of American chemical Society, (JACS)134, 17722-17730.

* – Author’s equal contribution

 

Ongoing Research:

Absorption of selected pesticide residues By plants

Publications:

 

  1. DT Abeysinghe, KAH Kumara, KAD Kaushalya, UG Chandrika, D Alwis. 2021. Phytochemical screening, total polyphenol, flavonoid content, in vitro antioxidant and antibacterial             activities of Sri Lankan varieties of Murraya koenigii and Micromelum minutum, Heliyon 7 (7), e07449
  2. DT Abeysinghe, D Alwis, KAH Kumara, UG Chandrika. 2021. Nutritive Importance and Therapeutics Uses of Three Different Varieties (Murraya koenigii, Micromelum minutum, and Clausena indica) of Curry Leaves: An Updated Review. Evidence-Based Complementary and Alternative Medicine 2021. https://doi.org/10.1155/2021/5523252
  3. IBK Thomas, KAP Gaminda, CD Jayasinghe,DT Abeysinghe, and R. Senthilnithy. DNAzymes, Novel Therapeutic Agents in Cancer Therapy: A Review of Concepts to Applications. Journal of Nucleic Acids 2021 https://doi.org/10.1155/2021/9365
  4. Ananda K Ghosh,Thelma Abeysinghe, Amnon Kohen,Altered Protein Dynamics Modified the Chemical Step in Thymidylate Synthase, Annual meeting of the American Society for Biochemistry and Molecular Biology, Chicago, USA. April 22-26th, 2017
  5. Thelma Abeysinghe, Catherine Suchanek, Amnon Kohen,Experimental Studies of Enzyme Catalyzed Hydrogen-Transfer Reactions, The 34th Midwest Enzyme Chemistry Conference Proceedings, pp. 22, North Western University, Illinois, USA. September 27th, 2014
  6. Catherine Suchanek,Thelma Abeysinghe, Amnon Kohen,Initial Velocity Studies Comparing Heavy and Light Thymidylate Synthase,12th Annual College of Engineering research Open House Conference Proceedings, pp. 17, The University of Iowa, USA April 10th, 2014
  7. Catherine Suchanek,Thelma Abeysinghe, Amnon Kohen,Method of Using Fast Cloning Technique to Express Thymidylate Synthase with Polyhistidine-tag to Aide in the Extraction of a Pure Protein, 13th Annual Meeting of American Institute of Chemical Engineers (AIChE), pp. 23, Hilton San Francisco Union Square, San Francisco, CA, USA November3-8th, 2013
  8. Thelma Abeysinghe, Amnon Kohen,Effects of a remote mutation on different chemical steps catalysed by Thymidylate Synthase, 22nd Annual Biocatalysis and Bioprocessing Conference Proceedings, pp. 34, The University of Iowa, USA October 21-22nd, 2013
  9. Thelma Abeysinghe, Zhen Wang, Amnon Kohen,Histidine 147 and its implications on the mechanism of Thymidylate Synthase, Holden Comprehensive Cancer Centre Research Retreat Proceedings, pp. 112, The University of Iowa, USA. June 13th, 2013
  10. Thelma Abeysinghe, Amnon Kohen,the role of protein’s long range dynamics transfer in Thymidylate Synthase catalysed reactions, The 33rd Midwest Enzyme Chemistry Conference Proceedings, pp. 21, Loyola University, Chicago, USA. October 12th, 2013
  11. Thelma Abeysinghe, Zhen Wang, Amnon Kohen,Effects of a Remote Mutation on the hydride transfer step in Thymidylate Synthase ,47th American Chemical Society (ACS) Midwest Regional Conference Proceedings, pp. 32, Omaha, USA, October 24-27th, 2012
  12. Thelma Abeysinghe, Zhen Wang, Amnon Kohen,Effects of Y209W on the hydride transfer step catalysed in Thymidylate Synthase, Holden Comprehensive Cancer Centre Research Retreat Proceedings, pp. 12, The University of Iowa, USA. June 14th, 2012
  13. Thelma Abeysinghe, Zhen Wang, Amnon Kohen,Long Range Protein Effects of Thymidylate Synthase, The 31st Midwest Enzyme Chemistry Conference Proceedings, pp. 18, University of Chicago, Chicago, USA. October 15th, 2011
  14. Thelma Abeysinghe, Zhen Wang, Amnon Kohen,Purification of R-6xH] – N5, N10 –methylene-5,6,7,8-tetrahydrofolate for studies of Thymidylate Synthase, The 30th Midwest Enzyme Chemistry Conference Proceedings, pp. 17, North Western University, Illinois, USA. October 16th, 2010
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